SS4.13 Water Quality of Lakes, Rivers and Coastal Zones
Date: Thursday, June 13, 2002
Time: 11:00:00 AM
Location: Carson B
 
HoldenP, Donald Bren Sch. Environ. Sci. & Mgmt., Univ. of California, Santa Barbara, USA, holden@bren.ucsb.edu
LaMontagne, M, G, Donald Bren Sch. Environ. Sci. & Mgmt., Univ. Calif., Santa Barbara, USA, 
 
URBANIZATION AND COASTAL WATER QUALITY: WHAT CAN MOLECULAR FINGERPRINTING TELL US?
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Human development of coastlines changes the quantity and quality of freshwater input to coastal waters. Overland flow of stormwater runoff and subsurface groundwater transport can alter watershed waters chemically and biologically, and both may affect the phylogenetic diversity and composition of coastal creek aquatic communities. We asked, what is the relationship between human development and bacterial communities in a coastal creek in Santa Barbara, CA? We also asked if the coastal lagoon harbored a bacterial community distinct from up or downstream. By terminal restriction fragment length polymorphism analysis of 16S rDNA amplified from creek, lagoon and ocean samples, we determined that 1) a development gradient in bacterial community composition appears in creek water, and 2) the lagoon and creek bacterial communities were similar in wet weather. Our work suggests that fingerprinting whole bacterial communities is a sensitive, accurate and reproducible approach for relating urbanization to watershed and coastal water quality.