CS39A Zooplankton - Feeding, Reproduction, Growth and Molecular Diversity
Date: Tuesday, June 11, 2002
Time: 3:15:00 PM
Location: View Royal
 
GarciaRL, University of Texas at El Paso, El Paso, USA, chibilinda@hotmail.com
Walsh, E, J, University of Texas at El Paso, El Paso, USA, ewalsh@utep.edu
 
IT'S ALL IN THE GENES: ASSESSMENT OF A COSMOPOLITAN ROTIFER SPECIES, PLATIONUS PATULUS
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The rotifer Plationus patulus is a common member of the planktonic community. As a basal member of the food web, it plays an important role in nutrient cycling and provides food for higher trophic levels. Recently, Plationus patulus was removed from the genus Brachionus based on morphological characters. The goal of this project is to use genetic data to determine the placement of this taxon in the phylogeny of the Rotifera. Three genes (ITS, E23 helix of the 18S nuclear ribosomal gene, and 16S mitochondrial gene) will be used for this analysis. To obtain these gene sequences, DNA will be amplified using PCR, purified, and sequenced using a Li-COR sequencer. Data will be analyzed using AlignIR and Eseq programs (LI-COR) before phylogenetic analyses using PAUP. Outgroups will consist of 5 species of Brachionus. We will include 5 geographically separated populations of P. patulus in the ingroup. The phylogenetic placement and proper identification of species is necessary to correctly interpret ecological data in limnological studies.