SS2.06 Microbial Diversity in Time and Space
Date: Tuesday, June 11, 2002
Time: 10:15:00 AM
Location: Lecture Theatre
 
Nierzwicki-BauerSA, Darrin Fresh Water Institute, Rensselaer Polytechnic Institute , Bolton Landing, NY, , USA, nierzs@rpi.edu
Vescio, P, A, Darrin Fresh Water Institute, Rensselaer Polytechnic Institute , Bolton Landing, NY,, USA, vescip@rpi.edu
Ostrofsky, E, B, Darrin Fresh Water Institute, Rensselaer Polytechnic Institute, Bolton Landing, NY,, USA, ostroe@rpi.edu
 
CHARACTERIZATION OF SURFACE MICROLAYER AND BULK WATER MICROBIAL COMMUNITIES IN A FRESHWATER LAKE IN THE ADIRONDACKS, NY
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Microbial community structure of the surface microlayer (thirty to sixty microns) and bulk water collected from multiple locations in a freshwater lake (Lake George, NY) was investigated in order to better understand the dynamics of this ecosystem. Samples were analyzed by phospholipid fatty acid analysis (PLFA), community 16S rDNA sequence analysis, and fluorescent in-situ hybridization (FISH). All samples contained diverse bacterial communities, with overall bacterial profiles clustering by sampling location opposed to microlayer versus bulk water. 16S rDNA sequence analysis revealed diverse bacterial communities, with a high proportion of clones from the Cytophaga/Flavobacter/Bacteroides (CFB), High G + C, and beta proteobacterial groups. Many sequences were closely related to clones from the Columbia River as well as other Adirondack lakes. Sequences falling within the Rubrivivax subgroup of the beta-proteobacteria were found solely in microlayer samples. Total cell counts were similar for microlayer and bulk water samples, however the microlayer samples contained a higher percentage of viable bacteria (determined by counts using a universal 16S rRNA eubacterial probe).