SS4.07 Lipids/Fatty Acids in Ecological Research
Date: Tuesday, June 11, 2002
Location: Poster Session - VCC
 
Parrilla Deborah, University of Puerto Rico-Department of Marine Sciences, La Parguera, Lajas, , parrilladeb@yahoo.com
 
ROLE OF STRESS IN EXPRESSION OF EICOSANOIDS IN THE RED ALGAE MURRAYELLA PERICLADOS (C. AGARDH)SCHMITZ
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The compound 12 hydroxyeicosatetraenoic acid (12-HETE) have been isolated from lipid soluble extracts from the benthic marine red alga Murrayella periclados . Eicosanoids and related polyunsaturated fatty acids (PUFA's) have a wide distribution in the marine environment In this study the lipid extracts from Murrayella was evaluated at 5, 10, 30 and 60 minutes after been exposed to combinations of temperature (10 to 45 oC) and salinities (10,25 and 65 o/oo), to examine the effects on 12-HETE concentration and to observed the expression of heat shock proteins (Hsp). Values of 12-HETE range from 0.02 to 0.045 mg/g of dry algal weight in response to temperature and salinity treatments. Values of 12-HETE start to increase after 10 minutes of exposure in both treatments. The presence of Hsp 70 and Hsp 25 was detected in samples that were immediately extract after been exposed during 10 minutes at 10 and 65 o/oo and at 45oC.