SS2.06 Microbial Diversity in Time and Space
Date: Tuesday, June 11, 2002
Time: 11:00:00 AM
Location: Lecture Theatre
ComeauAM, Microbiology and Immunology, University of British Columbia, Vancouver, Canada,
Suttle, C, A, Earth and Ocean Sciences, Botany, Microbiology and Immunology, University of British Columbia, Vancouver, Canada,
Vibrio parahaemolyticus is a marine bacterium that causes gastroenteritis from the consumption of raw or undercooked shellfish. We are interested in using viruses infecting this bacterium as models for examining the diversity of natural viral communities and the role of viruses in structuring marine microbial diversity. Bacterial and viral strains were isolated from water column, sediment pore water, and oyster homogenate samples from inlets adjacent to the Strait of Georgia during 2000 and 2001. Host range/sensitivity studies were performed on a subset of the isolates (n=17 bacteria/18 viruses) to discern phenotypic diversity between the sample mediums. A version of repetitive-element PCR (ERIC-PCR) was also used to describe the genetic diversity of the bacterial isolates. Oyster and water column bacteria separated into two major phenotypic clusters, with the sediment bacteria interspersed among them. This relationship was also reflected in the ERIC-PRC patterns of the bacteria. In contrast, all viral isolates exhibited similar patterns of infection and could not be separated into distinct groups.