SS2.06 Microbial Diversity in Time and Space
Date: Tuesday, June 11, 2002
Time: 9:45:00 AM
Location: Lecture Theatre
 
OrtmannAC, University of British Columbia, Vancouver, Canada, ortmann@interchange.ubc.ca
Suttle, C, A, University of British Columbia, Vancouver, Canada, csuttle@eos.ubc.ca
 
GENETIC DIVERSITY WITHIN A DEEP-SEA HYDROTHERMAL PLUME
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The stable hydrothermal plume associated with the Main Field of the Endeavour Ridge in the northeast Pacific originates from rapidly venting black smokers at a depth of 2200 m. The fluid emitted from these vents rises quickly, mixing with the cold surrounding seawater, changing chemically and physically. The buoyant fluid rises up and begins to spread laterally with the currents. Samples were obtained from within the vent field using ROPOS in May of 2001. CTD samples of the vertical structure of the plume as well as overlying water were collected in July of 2000 and 2001 along a SW transect. DNA extracted from these water samples was amplified using primers designed to amplify the 16S gene from most bacteria and archaea. DGGE was used to construct community fingerprints of the prokaryotic community. The samples represent temperatures of 1.8 to 80 C from depths ranging from 1500 m to 2500 m. Community fingerprints indicate that the diversity of prokaryotes changes over short time scales in rapidly evolving environments.