CS16 Harmful Algal Blooms
Date: Wednesday, June 12, 2002
Location: Poster Session - VCC
 
PedersonBA, Mote Marine Laboratory, Sarasota, USA, pederson@mote.org
Kirkpatrick, G, J, Mote Marine Laboratory, Sarasota, USA, gkirkpat@mote.org
Berg, B, A, Mote Marine Laboratory, Sarasota, USA, barbib@mote.org
Haywood, A, J, Florida Marine Research Institute, St. Petersburg, USA, Allison.Haywood@fwc.state.fl.us
Higham, C, J, Mote Marine Laboratory, Sarasota, USA, chigham@mote.org
 
DIFFERENTIATING TWO FLORIDA HARMFUL ALGAL BLOOM SPECIES USING HPLC PIGMENT CHARACTERIZATION
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A large scale bloom of Karenia brevis was identified on the west Florida continental shelf during the ECOHAB process cruise conducted September 20–26, 2001. Offshore of the large bloom a small isolated patch of morphologically distinct K. brevis-like cells was also found. Following the HPLC methods of Wright et. al.(1990), filters collected at both inshore and offshore bloom locations were analyzed for algal pigment content. Results indicate that the offshore patch was distinct in relation to the gyroxanthin-diester to chlorophyll a ratio as well as the amount of fucoxanthin per cell. Bloom data from previous years have shown the gyroxanthin-diester/chl a ratio to be constant for Karenia brevis and studies comparing multiple Karenia species demonstrated a variation in both gyroxanthin-diester/chl a and fucoxanthin per cell. The distinct pigment signature coupled with the morphological differences observed microscopically suggest the offshore patch may have been comprised of a Karenia species other than K. brevis.