SS2.03 Phytoplankton Ecology Using Molecular Approach
Date: Thursday, June 13, 2002
Location: Poster Session - VCC
 
ThamatrakolnK, Scripps Institution of Oceanography, La Jolla, USA, kthamatr@ucsd.edu
Latz, M, I, Scripps Institution of Oceanography, La Jolla, USA, mlatz@ucsd.edu
 
MOLECULAR STUDIES ON THE EFFECT OF FLOW ON DIATOMS AND DINOFLAGELLATES
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Diatoms and dinoflagellates respond differentially to flow, which enhances growth rates in diatoms and inhibits it in dinoflagellates. A molecular and biochemical approach was used to study the cellular effects of flow on the diatom, Phaeodactylum tricornutum and the dinoflagellate, Lingulodinium polyedrum. Expression of certain heat shock proteins (Hsp) is a highly conserved response to stress. Inducible Hsp70 expression was analyzed by Western blot in cultures exposed to flow. Patterns of expression differed from still controls in both organisms. Subtractive hybridization was used to identify genes expressed in response to flow. Libraries were screened to confirm differential expression and positive clones were sequenced and compared against known sequences using BLAST. These genes will be further characterized and may prove useful as genetic markers for physiological stress. These results demonstrate that subtraction is a useful tool for identifying differentially expressed genes in phytoplankton and may be applicable for isolating genes induced by other environmental stresses.