SS2.06 Microbial Diversity in Time and Space
Date: Tuesday, June 11, 2002
Location: Poster Session - VCC
 
MilbrandtEC, Oregon Institute of Marine Biology, Charleston, OR, USA, emilbran@oimb.uoregon.edu
Shapiro, L, P, Oregon Institute of Marine Biology, Charleston, OR, USA, lshapiro@oimb.uoregon.edu
 
SPATIAL VARIABILITY AND COMMUNITY COMPOSITION PATTERNS OF BACTERIA IN SOUTH SLOUGH ESTUARY, OR, USA
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Molecular methods and data reduction techniques were used to describe sediment 16S rDNA Bacteria communities along two natural gradients:, depth and the presence of algal mats. Since depth is known to have strong redox gradients, it was hypothesized that the redox environment strongly selects for a unique Bacterial community. The presence of algal mats was hypothesized to select for a unique community due to their influence on underlying sediments. Replicate sediment samples (4) were fractionated by depth in the presence and absence of algal mats. DGGE was used to separate the 16S rDNA community and sequencing was used to identify its members. Each site showed evidence of community differences by depth, and between mat and non mat samples. However, the patterns of difference in both depth comparisons and mat to non mat comparisons are unique to each site. This suggests that each site has a unique suite of characteristics that select for the presence or absence of populations in community, and within each site, depth and the presence of algal mats are important in selecting for community structure.