SS2.02 Biogeochemical Process at the Sediment-Water Interfaces
Date: Tuesday, June 11, 2002
Time: 10:00:00 AM
Location: Carson B
 
SlivaL, University of Toronto, Scarborough, Canada, sliva@utsc.utoronto.ca
Williams, D, D, University of Toronto, Scarborough, Canada, williamsdd@utsc.utoronto.ca
Fulthorpe, R, R, University of Toronto, Scarborough, Canada, fulthorpe@utsc.utoronto.ca
 
EXPLORATION OF THE LINK BETWEEN BACTERIAL COMMUNITIES AND SUBSURFACE HYDROLOGY IN THE HYPORHEIC ZONE OF THE SPEED RIVER, ONTARIO, CANADA
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Bacteria likely play an important role in a broad range of metabolic processes that occur within the hyporheic zone and that provide nutrients for interstitial invertebrates. As a first step in understanding this role we examined spatial relationships among bacterial types and their activity, and subsurface hydrology. Bacterial community fingerprints, at various substrate depths, in upwelling and downwelling areas were compared through optimisation and application of Terminal-Restriction Fragment Length Polymorphism (T-RFLP), a PCR-based molecular method that uses ribosomal RNA genes (rDNA) to determine compositional similarities or differences among various bacterial communities. These data were gathered both from undisturbed substrates and from an in situ manipulation experiment. Initial findings show a greater similarity in bacterial communities among depths than between upwelling and downwelling areas.