CS16 Harmful Algal Blooms
Date: Wednesday, June 12, 2002
Location: Poster Session - VCC
 
HighamCJ, Mote Marine Laboratory, Sarasota, FL, USA, chigham@mote.org
Kirkpatrick, G, J, Mote Marine Laboratory, Sarasota, FL, USA, 
Pederson, B, A, Mote Marine Laboratory, Sarasota, FL, USA, 
Berg, B, A, Mote Marine Laboratory, Sarasota, FL, USA, 
 
PHOTOPIGMENT CONTENT OF THREE KARENIA BREVIS CLONES IN RESPONSE TO VARYING LIGHT LEVELS
image
The photopigment content of three clones of the Florida (USA) red-tide dinoflagellate Karenia brevis (Davis) G. Hansen & Moestrup, isolated from natural bloom events in 1953, 1996, and 2001were analyzed after acclimating cultures to high and low irradiance treatments. Photosynthetic pigment concentrations in each clone decreased with increasing irradiance. Photosynthetic and photoprotective pigment concentrations increased with increasing years a clone has been in culture. However, the gyroxanthin-diester/chlorophyll a ratios were equivalent among the clones and light treatments. The fucoxanthin/chlorophyll a ratios did not demonstrate a similar equivalence. The gyroxanthin-diester/chlorophyll a ratio could be considered a unique ‘signature’ and incorporated into future pigment-based monitoring applications to allow for the detection of Karenia brevis and differentiation between Karenia species. Previous light history and the time a clone has been in culture should be considered when comparing culture and natural bloom photopigments.