SS2.03 Phytoplankton Ecology Using Molecular Approach
Date: Thursday, June 13, 2002
Time: 4:45:00 PM
Location: Colwood
 
CrosbieND, Institute for Limnology, Mondsee, Austria, nick.crosbie@oeaw.ac.at
 
FLOW CYTOMETRIC MAPPING AND ISOLATION OF FRESHWATER AUTOTROPHIC PICOPLANKTON
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Flow cytometric methods (FCM) have not been applied to the same extent in limnology as in oceanography. I discuss the potential reasons for this, then 1) show how FCM were of considerable utility to a study on the distribution and seasonal patterns of autotrophic picoplankton (picoeukaryotes, solitary and colonial picocyanobacteria, and cells dislodged from colonial picocyanobacteria through physical and biological processes) inhabiting pre-alpine lakes of the 'Salzkammergut' region (Austria), and 2) discuss isolation of freshwater autotrophic picoplankton by sorting single cells or colonies into microwells containing growth media: subsequent genetic, microscopic and flow cytometric analysis of isolates thus obtained has revealed a considerable diversity in pigment type and morphology. Finally, I briefly discuss current strengths and weaknesses in software and hardware from the perspective of the oceanographer or limnologist applying FCM in his/her research.