SS2.06 Microbial Diversity in Time and Space
Date: Tuesday, June 11, 2002
Location: Poster Session - VCC
 
WarneckeF, Max Planck Institute for Marine Microbiology, Bremen, Germany, fwarneck@mpi-bremen.de
Pernthaler, J, , Max Planck Institute for Marine Microbiology, Bremen, Germany, 
Alonso, C, , Max Planck Institute for Marine Microbiology, Bremen, Germany, 
Amann, R, I, Max Planck Institute for Marine Microbiology, Bremen, Germany, 
 
A SCREENING PROTOCOL FOR THE RAPID DETECTION OF INDIVIDUAL PHYLOGENETIC GROUPS IN BACTERIOPLANKTON 16S RIBOSOMAL DNA CLONE LIBRARIES
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We developed a PCR-based protocol for the rapid screening of large environmental 16S rDNA clone libraries for particular bacterial sequence types. Specific primer pairs were constructed for two phylogenetic lineages, and the accumulation of PCR product was followed using an ABI SDS 7700 instrument and the SYBR Green I nucleic acid stain. By this procedure we were able to rapidly obtain >30 different actinobacterial 16S rDNA sequences from the bacterioplankton of various lakes, although this subgroup on average constituted <5% of clones in the libraries. We then proceeded to a comparative screening for differences between freshwater and marine members of the Cytophaga/ Flavobacterium/ Bacteroides phylum (CFB). Altogether, >1000 clones from libraries of lake, brackish water and coastal North Sea picoplankton were analyzed.