SS4.05 Tribute to Thomas Frost
Date: Monday, June 10, 2002
Time: 2:45:00 PM
Location: Sidney
HurleyJP, University of Wisconsin, Madison, WI, USA,
Garrison, P, J, Wisconsin Department of Natural Resources, Monona, WI, USA,
Cleckner, L, B, University of Wisconsin, Madison, WI, USA, 
Aquatic Pigments as Indicators of Plankton and Periphyton Composition: From Northern Wisconsin to the Florida Everglades
High performance liquid chromatography (HPLC) coupled with photodiode array (PDA) detection has proven to be an effective means of determining the composition of plankton and periphyton in lake and wetland systems. Using this technique, complex layers of deep plankton and phototrophic bacteria were identified in several northern Wisconsin lakes that develop seasonal anoxia. We have also identified the both the presence of complex layering of chlorophylls and carotenoids in periphyton and diurnal movement of phytoplankton and phototrophic bacteria of the Florida Everglades. Presence of bacteriochlorophylls in periphyton mats, indicative of intense sulfur cycling, correlates with methylation of mercury. In periphyton, we also observe the presence of a UV protectant pigment that without PDA, would probably be identified as the carotenoid fucoxanthin. Presence of the UV pigment is dependent on both dissolved organic carbon quantity and quality.