SS4.07 Lipids/Fatty Acids in Ecological Research
Date: Tuesday, June 11, 2002
Time: 10:00:00 AM
Location: Saanich
 
GreensladeNJ, University of Essex, Colchester, United Kingdom, njgree@essex.ac.uk
Ball, A, S, University of Essex, Colchester, United Kingdom, andrew@essex.ac.uk
Smith, D, J, University of Essex, Colchester, United Kingdom, djsmitc@essex.ac.uk
 
THE USE OF STEROLS TO DEVELOP A NEW STYLE OF ARTIFICIAL TRACER IN AQUATIC SYSTEMS
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PLA 600 is a water-soluble derivative of lanosterol, developed to combine sterol profile analysis and artificial tracing in estuarine environments, using current sterol detection methods. The solubility of PLA 600 is 69 mg/l and suspensions of up to 100,000 mg/l have been made. Current work indicates that PLA 600 interacts with marine particulates in the same manner as other sterols. Sediment and water sampling downstream from the point of introduction was carried out using glassware with PTFE lined lids and sterol extraction followed Mudge & Norris (1997). Saponification with potassium hydroxide removes the side chain conferring PLA 600 solubility, enabling detection of the sterol and quantification typically by Gas Chromatography Mass Spectrophotometry (GC-MS). The use of thin layer chromatography (TLC) for field screening is currently under investigation. The development of PLA 600 is particularly for use in environmentally sensitive waters. In areas of aquaculture, it is becoming increasingly difficult to trace sources of faecal contamination due to restrictions in the use of existing tracing techniques.