BOXWELL, J. A.. COLLEGE OF WILLIAM AND MARY, 804-684-7250
CANUEL, E. A.. SCHOOL OF MARINE SCIENCE, 804-684-7250
APPLICATIONS OF MICROWAVE-ASSISTED SOLVENT EXTRACTION (MASE) FOR ISOLATING LIPID BIOMARKER COMPOUNDS ASSOCIATED WITH ESTUARINE PARTICULATE MATTER
Over recent years, microwave applications have become widespread in the field of chemistry. Chemists are currently using microwave energy to catalyze reactions during chemical synthesis, to study molecular properties, and to aid in sample preparation (e.g., digestion, extraction and separation). We have been developing microwave assisted extraction techniques for the isolation of lipid biomarker compounds from estuarine seston and sediments. The technique offers several advantages including a reduction in sample processing time, higher sample throughput, decreases in solvent use that result in financial savings both in terms of solvent consumption as well as waste disposal fees, and an increase in laboratory safety by reducing one's exposure to toxic solvents.
In this presentation, we will outline our efforts to develop a method for applying MASE to estuarine samples. First, we determined recoveries for standards spiked to glass fiber filters. The standards include representive compounds from three compound classes commonly analyzed in our laboratory fatty acids, alcohols and sterols). We then quantified recoveries for lipid biomarker compounds extracted from a surface sediment sample collected from the Chesapeake Bay. Our results document that recoveries obtained using MASE are comparable to those obtained using solvent extraction assisted by sonication.
Day: Wednesday, Feb. 3
Location: Sweeney Center