Pacheco-Bernier, L. M. College of Marine Studies, University of Delaware, Lewes, lizapacheco@hotmail.com
Ullman, W. M. College of Marine Studies, University of Delaware, Lewes, ullman@udel.edu

 
OPTIMUM CONDITIONS FOR CALCIUM CARBONATE PRODUCTION BY THE COCCOLITHOPHORIDS PLEUROCHRYSIS CARTERAE AND EMILIANIA HUXLEYI.
 
The marine coccolithophorids are a group of planktonic calcium carbonate secreting microalgae. The caco3 PRODUCED BY THESE ORGANISMS MAY BE USEFUL AS A LEAD-FREE DIETETIC SUPPLEMENT FOR HUMANS ANALOGOUS TO THE FOOD SUPPLEMENT TUMSTM. EXPERIMENTS TO DETERMINE THE OPTIMUM CULTURE CONDITIONS FOR THE PHOTOSYNTHETIC PRODUCTION OF THE COCCOLITHOPHORID, PLEUROCHRYSIS CARTERAE AND EMILIANIA HUXLEYI, AND BIOGENIC CACO3 WERE PERFORMED IN BATCH CULTURE AT 16 OC AND 22OC RESPECTIVELY AND WITH A VARIABLE NITRATE (NO3-) AND PHOSPHATE (PO4-3) CONCENTRATIONS AND RATIOS. FOR P CARTERAE THE INITIAL (LOG) STAGE OF GROWTH PRODUCED THE MOST CALCIUM CARBONATE. AFTER THREE DAYS IN BATCH CULTURE, THERE WAS ONLY A SMALL INCREASE IN CACO3 PRODUCTION, EVEN IF NUTRIENT CONCENTRATIONS WERE SUFFICIENT FOR CONTINUED ALGAL GROWTH. THIS SAME PATTERN WAS OBSERVED IN EMILIANIA HUXLEYI. FOR BOTH KIND OF COCCOLITHOPHORIDS OPTIMUM PRODUCTION OF CACO3 REQUIRED A MINIMUM OF 50 mM NO3- CONCENTRATION. THE OPTIMUM PO4-3 CONCENTRATION COULD NOT BE DETERMINED, AS PHOSPHORUS HAD BEEN STORED FROM PREVIOUS CULTURE MEDIUM. THE OPTIMUM DESIGN OF A FLOW-THROUGH REACTOR FOR THE PHARMACEUTICAL PRODUCTION OF BIOGENIC CACO3 WOULD HAVE A RESIDENCE TIME OF 3 DAYS, BE BRIGHTLY LIT, WELL MIXED USING A STIRRING DEVICE COUPLED TO A SETTLING TUBE FOR THE COLLECTION OF THE SOLID PRODUCT.
 
Day: Wednesday, Feb. 3
Time: Poster
Location: Sweeney Center
 
Code: SS54WE0039S