Rublee, P. A. University of North Carolina at Greensboro, rublee@uncg.edu
Kempton, J. University of North Carolina at Greensboro, jwkempto@goodall.uncg.edu
Schaefer, E. University of North Carolina at Greensboro,
Oldach, D. Institute of Virology, oldach@umbi.umd.edu
Burkholder, J. M. North Carolina State University, joanne_burkholder@ncsu.edu
Glasgow, H. B. North Carolina State University, howard_glasgow@ncsu.edu

 
GENE PROBES TO PFIESTERIA PISCICIDA: RESULTS FROM THE 1998 ACTIVE SEASON.
 
Three types of gene probe methods were developed for rapid identification of P. piscicicda in cultures and field samples: 1) direct PCR to detect fragments of the Pfiesteria piscicida small subunit ribosomal DNA (SSU rDNA) gene; 2) heteroduplex mobility assays to compare SSU rDNA amplicons from field samples and cultures to culture standards; and 3) fluorescent in situ hybridization with SSU rDNA probes. The methods were applied to culture isolates and field samples during summer and fall of 1998, including field samples from fish kill events (Neuse River Maryland) and fish lesion events (Shiles Creek, MD). All three methods confirmed the presence of Pfiesteria piscicida at fish kill and fish lesion events and suggested widespread distribution of P. piscicicda at sublethal concentrations in enriched estuarine environments. The presence of such "silent" populations suggests, in turn, that these systems are at risk for fish disease, fish kills, and human health impacts if natural or anthropogenic stresses increase.
 
Day: Friday, Feb. 5
Time: 04:45 - 05:00pm
Location: Eldorado Hotel
 
Code: SS42FR0445E