Alfrieder, A. . University of South Florida, alfreider@seas.marine.usf.edu
Paul, J. H.. University of South Florida, jpaul@seas.marine.usf.edu

 
GENETIC DIVERSITY OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE IN MARINE PHYTOPLANKTON INVESTIGATED BY DENATURING GRADIENT GEL ELECTROPHORESIS OF PCR-AMPLIFIED GENE FRAGMENTS
 
Marine phytoplankton are known to fix carbon dioxide primarily through the action of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), the first enzyme in the Calvin cycle. To investigate the diversity of the RubisCO gene, partial gene sequences of the RubisCO large subunit DNA (rbcL) from cultures with different evolutionary lineages were amplified by PCR using degenerate primers, and sequence heterogeneity was studied by denaturing gradient gel electrophoresis (DGGE) analysis. The investigation of PCR amplified rbcL nucleic acids extracts from natural phytoplankton communities by DGGE, cloning and sequence analysis are in progress.
 
Day: Tuesday, Feb. 2
Time:
Location: Sweeney Center
 
Code: SS41TU1547S