Sullivan, J. B.. University of Georgia, jsulliva@arches.uga.edu
Chen, F. B.. University of Georgia, fchen2@arches.uga.edu
Hodson, R. E.. University of Georgia, rhodson@arches.uga.edu

 
CONSTITUTIVE PRODUCTION OF NITRITE REDUCTASE IN PSEUDOMONAS STUTZERI DETECTED BY IN SITU RT-PCR
 
Reduction of oxidized forms of nitrogen (i.e. nitrate, nitrite, nitric oxide, and nitrous oxide) to dinitrogen within the global nitrogen cycle is caused, biologically, only by denitrification. Our goal is to gain a clearer understanding of the impact of bacterial community structure and function on denitrification, which is solely a procaryotic process. In order to determine conditions of enzyme expression, an experiment to detect the presence of nitrite reductase mRNA encoded by the gene nirS inside intact bacterial cells was undertaken. The method chosen was in Situ Reverse Transcription and Polymerase Chain Reaction (in Situ RT-PCR). Cells from a marine denitrifier, Pseudomonas stutzeri (ATCC 14405), were grown aerobically without nitrate and anaerobically in the presence of nitrate. Given the supposedly inducible nature of the system, the aerobically grown cells should have been free of nirS mRNA. Whereas cells grown without oxygen should have been positive for the transcript. The results of the experiment showed production of mRNA for both aerobic and anaerobic cells with suitable negative controls. The results suggested constitutive production of nirS mRNA.
 
Day: Tuesday, Feb. 2
Time: Poster
Location: Sweeney Center
 
Code: SS41TU0919S