Stürmeyer, H. H. Max-Planck-Institute for Marine Microbiology, hstuerme@mpi-bremen.de
Pernthaler, J. H. Max-Planck-Institute for Marine Microbiology, jperntha@mpi-bremen.de
Glöckner, F. FO. Max-Planck-Institute for Marine Microbiology, fgloeckn@mpi-bremen.de
Amann, R. R. Max-Planck-Institute for Marine Microbiology, ramann@mpi-bremen.de

 
DIVERSITY AND COMMUNITY STRUCTURE OF HETEROTROPHIC BACTERIOPLANKTON IN THE NORTH SEA
 
Bacterioplankton is recognized as an important agent of biogeochemical change in marine ecosystems. Yet little is known about the bacterial species that make up pelagic microbial communities and their spatial and temporal dynamics. Uncertainties about the diversity and structure of natural bacterioplankton communities originate from the limitations of standard microbiological cultivation techniques. Cultivation independent characterization of naturally occurring microorganisms via 16S rRNA cloning and sequencing indicated the presence of several major bacterial groups: the cyanobacteria, proteobacteria of the a- and g-subclass and members of the Cytophaga/Flavobacterium cluster. In our study, the diversity and structure of the heterotrophic microbial community in the surface layer of the North Sea near Helgoland was investigated. Classical microbiological techniques that involve the study of pure cultures and modern molecular approaches based on analysis of rRNA sequences were combined. Initial comparision of 16S rRNA clones from environmental DNA and directly amplified 16S rDNA of isolates was done by denaturing gradient gel electrophoresis (DGGE) and amplified ribosomal DNA restriction analysis (ARDRA) patterns. Group and genus specific nucleic acid probes were designed based on these 16S rDNA sequences and used for screening of isolates and clones and for determination of abundances by in situ hybridization of bacterioplankton over the year.
 
Day: Tuesday, Feb. 2
Time: 09:00 - 09:15am
Location: Hilton of Santa Fe
 
Code: SS40TU0900H