Keough, B. P.. University of Minnesota-Duluth, bkeough@d.umn.edu
Hicks, R. P.. University of Minnesota-Duluth, rhicks@d.umn.edu

 
DISTRIBUTION OF ARCHAEAL NUCLEIC ACIDS IN PICOPLANKTON FROM GREAT LAKES OF THE WORLD
 
Keough, B. P., University of Minnesota-Duluth, Duluth, MN 55812, USA, bkeough@d.umn.edu Hicks, R. E., University of Minnesota-Duluth, Duluth, MN 55812, USA, rhicks@d.umn.edu DISTRIBUTION OF ARCHAEAL NUCLEIC ACIDS IN PICOPLANKTON FROM GREAT LAKES OF THE WORLD Although bacteria often dominate picoplanktonic communities in aquatic environments, the biodiversity of these communities is poorly understood. Quantitative hybridization with 16S rRNA-based probes targeting the three major Domains of life, Bacteria, Eucarya, and Archaea, was used to assess the diversity of nucleic acids extracted from water samples taken in the North American Great Lakes during 1992. Up to two percent of the total nucleic acids were from the archaeal Domain, which is surprising because the known members of this Domain are typically associated with environmental extremes; hypersaline, strictly anoxic, or hyperthermal conditions. A second sampling cruise in 1993 was undertaken to verify this finding. Samples were also taken from two Russian great lakes (Lakes Ladoga and Onega) and one African great lake (Lake Victoria) between 1994-1996 to determine if archaeal nucleic acids in planktonic communities are a common feature of other great lakes of the world. Quantitative hybridization revealed archaeal nucleic acids in both the North American and world great lakes, which not only helped to confirm the presence of archaeal nucleic acids in the the North American Great Lakes, but also suggests that archaeons may be a common member of freshwater pelagic ecosystems. SS40 SS41 CS02: Bacteria Oral
 
Day: Tuesday, Feb. 2
Time: Poster
Location: Sweeney Center
 
Code: SS40TU0836S