Methe', B. A. University of Massachusetts-Amherst, bmethe@microbio.umass.edu
Zehr, J. A. Rensselaer Polytechnic Institute, zehrj@rpi.edu

 
COMPARISON OF BACTERIAL DIVERSITY IN ADIRONDACK LAKES BASED ON 16S RIBOSOMAL RNA AND DNA DERIVED SEQUENCES: IMPLICATIONS FOR RNA AND DNA-BASED AMPLIFICATION APPROACHES
 
Aquatic microbial communities are often studied by amplification and sequencing of 16S rDNA or 16S rRNA by PCR or RT-PCR, respectively. The two approaches have respective advantages and disadvantages and may provide different types of information. 16S rDNA has yielded extensive information on the composition of freshwater and marine microbial assemblages. The composition of bacterial communities of three Adirondack Mountain lakes were investigated by using both PCR and RT-PCR approaches. Analysis of 16S sequences showed that representatives of the same basic phylogenetic groups were obtained. All of the higher level phylogenetic classifications observed in the rDNA study were present in at least one of the three lakes in the rRNA study (Cytophaga-Flexibacter-Bacteroides, Verrucomicrobium, cyanobacteria and chloroplasts, Acidobacterium, high G+C gram positives and the alpha, beta and gamma Proteobacteria). These results are interesting, since the rDNA and rRNA analyses were performed in different years. The results strongly indicate that 1) both techniques provide information on the most important phylogenetic groups, 2) the phylotypes previously reported to be important in lakes (the beta proteobacteria) are synthesizing RNA and are not dead, and 3) lakewater bacterial communities are relatively stable or predictable, at least with respect to phylogenetic composition of the major groups.
 
Day: Tuesday, Feb. 2
Time: Poster
Location: Sweeney Center
 
Code: SS40TU0784S