Cambpell, B. J. University of Delaware, College of Marine Studies, bjc@udel.edu
Jeanthon, C. J. CNRS, Universitie Pierre et Marie Curie, jeanthon@sb-roscoff.fr
Prieur, D. CNRS, Universitie Pierre et Marie Curie, prieur@sb-roscoff.fr
Cary, C. University of Delaware, College of Marine Studies, caryc@udel.edu

 
PHYLOGENETIC ANALYSIS OF BACTERIAL SYMBIONTS OF ALVINELLA POMPEJANA, A EURYTHERMAL POLYCHAETE INHABITING EXTREME HYDROTHERMAL VENT ENVIRONMENTS
 
Alvinella pompejana is a polychaetous annelid that inhabits high temperature vent environments characterized by high concentrations of hydrogen sulfide and heavy metals. Alvinella is found associated with a dense, specific epibiotic microflora that inhabit the worm's dorsal integument. In past studies, we have characterized these bacteria by both cultivation as well as a molecular survey of 16S rRNA genes obtained from bulk DNA extracted from Alvinella. In this investigation, we characterized 153 bacteria isolated from the Alvinella microbial community. Sequences from these isolates were phylogenetically grouped with the predominant members of the clones obtained from the previously characterized 16S rDNA library. The majority of the bacterial isolates group within the gamma proteobacteria subdivision, and none with the epsilon. This is in stark contrast to the results obtained from the 16S rDNA library analysis, where over 80% of the clones are epsilons. These results support the generally accepted hypothesis that bacterial communities are composed of species that are not easily grown on common microbiological media. Only 1 clone from the 16S rDNA library was identical to any of the bacterial isolates and phylogenetically grouped with Acinetobacter. The importance of Acinetobacter in the Alvinella microbial community is resolved by in situ hybridization.
 
Day: Tuesday, Feb. 2
Time: Poster
Location: Sweeney Center
 
Code: SS27TU0724S