Rose, K. N. University of Guelph, rosek@uoguelph.ca
Hopcroft, R. N. Monterey Bay Aquarium Research Institute, hopcroft@mbari.org
Roff, J. C. University of Guelph, jroff@uoguelph.ca

 
FLUORESCENT EXOSKELETONS. A NOVEL METHOD FOR THE CALCULATION OF CRUSTACEAN GROWTH RATES.
 
Despite recent advances, there is an ongoing battle to establish methods for assessing secondary productivity in aquatic systems. We have developed a new fluorescent technique for determining growth rates in asynchronously developing populations of planktonic crustaceans. The non-toxic conjugate of the protein avidin and the dye rhodamine-B can be employed to label the chitinous exoskeleton of these organisms, and the addition of antibiotic slows bacterial degradation. Individual molt times can be determined by relabeling individuals through sequential molt cycles. Mixed assemblages of zooplankton can be easily stained in a laboratory or field setting, incubated, and then the stage specific durations can be determined for each species present. Initial studies performed on laboratory cultures of Daphnia magna have quite successfully differentiated between molted and unmolted individuals resulting in easily obtained individual development times. Field studies using marine copepods have also been attempted. This technique may simplify and improve field measurements of growth rates.
 
Day: Tuesday, Feb. 2
Time: 04:00 - 04:15pm
Location: Hilton of Santa Fe
 
Code: SS23TU0400H