Hassett, R. Ohio University, email@example.com
ANALYZING THE BIOCHEMICAL COMPOSITION OF INDIVIDUAL COPEPODS
Recent advances in the use of highly sensitive fluorescent substrates have provided the opportunity to analyze multiple biochemical compounds in individual copepods. To date, most measurements on copepods have relied on either using large numbers of individuals grouped together (losing information on the variability among individuals) or making a few measurements on single animals. The ability to make multiple measurement on a single animal can provide a useful tool for determining the physiological and nutritional condition of field-caught animals.
A protocol has been developed for analyzing biochemical constituents (protein, lipid, RNA, DNA), metabolic enzyme activities (citrate synthase (CS), carnitine palmitoyl transferase (CPT, rate-limiting enzyme in lipid B-oxidation), glutamate dehydrogenase), and digestive enzyme activities (protease, laminarinase) of individual Calanus finmarchicus CVs. A simple, highly sensitive assay has been developed for CS and CPT using a fluorescent probe that reacts with free CoA. Assay protocols have been devloped for both standard fluorometer cuvettes (3 ml volumes) and microwell plates (0.25 ml volumes) for most of the biochemical measurements. This approach is being used to investigate the variability in physiological condition among individual C. finmarchicus during overwintering in the Gulf of Maine.
Day: Wednesday, Feb. 3
Location: Sweeney Center