Baty, A. M. Center for Biofilm Engineering, ace_b@erc.montana.edu
Eastburn, C. M. Center for Biofilm Engineering, callie_e@erc.montana.edu
Techkarnjanaruk, S. The Flinders University of South Australia, s.techkarn@flinders.edu.au
Goodman, A. E. The Flinders University of South Australia, a.goodman@flinders.edu.au
Geesey, G. G. Center for Biofilm Engineering, gill_g@erc.montana.edu

 
THE FATE OF A CHITINOLYTIC MARINE BACTERIA ASSOCIATED WITH A PURE CHITIN THIN FILM IN A SEAWATER ENVIRONMENT
 
It is thought that the hydrolytic activity of microbial populations associated with detrital POM generates a significant amount of DOM in the open ocean. This DOM may contribute to the growth and reproduction of planktonic populations. This research uses thin films of pure chitin, as the sole carbon, nitrogen and energy source, to characterize the fate of a chitinolytic marine bacteria associated with this fraction of the organic matter pool. Up-expression of a chitin binding gene (chiB) is also quantified through the use of a gfp reporter system. The data showed that the chitin thin films supported a biofilm that reached a cell density of 8.0x10^6 cells/cm^2 and produced 6.0x10^5 cfu's/mL in the effluent. This shows that a pure chitin surface can directly displace an enormous amount of viable cells to the bulk aqueous phase as well as support a large surface associated population. Furthermore, up-expression for the chiB gene was localized within discrete regions in the biofilm. This suggests that much of the DOM produced by the up-expressed regions of the surface associated population may remain associated with the POM phase. Subsequently, this surface associated DOM may be utilized by those sub-populations that are not up-expressed.
 
Day: Monday, Feb. 1
Time: 02:45 - 03:00pm
Location: Sweeney Center
 
Code: SS17MO0245S