Bonner, J. S.. Texas A&M University-Corpus Christi, bonner@acs.tamu.edu
Mills, M. S.. U.S. EPA,
Simon, M. A.. Texas Engineering Experiment Station-Corpus Christi,
Autenrieth, R. JL.. Texas A&M University,
McDonald, T. J.. Texas Engineering Experiment Station-Corpus Christi,

 
BIODEGRADATION OF PETROLEUM IN A WETLAND
 
An intentional release of oil was conducted to assess bioremediation in a sensitive wetland environment. A randomized complete block design with six replicates per treatment was used to test three treatments, control, inorganic nutrients, and inorganic nutrients plus an alternate electron acceptor. Eighteen plots were oiled with a "weathered" Arabian light crude oil applied evenly onto the wetland sediment during low tide. Each plot consisted of a 5 m x 5 m perimeter and an elevated scaffold system to permit sampling and monitoring without impacting the study area. Sediment samples were analyzed for petroleum chemistry, inorganic nutrients, toxicity, and microbial counts. Petroleum concentrations were determined using gross composition analysis (TLC-FID), and target compound analysis (GC-MS). Normalizing to a conservative compound in the crude oil, 17 alpha , 21 beta-(H)hopane, was used to account for physical flushing and to reduce the influences of heterogeneity in the interpretation of the petroleum chemistry data. Target compound analyses indicated that inorganic nutrient additions accelerated biodegradation rates relative to the other treatments. There was greater than 95% degradation of target compounds found in the petroleum within an average of 167, 80, 118 days for the control, nutrient, and nutrient plus nitrate treatments, respectively.
 
Day: Monday, Feb. 1
Time: 02:30 - 02:45pm
Location: Hilton of Santa Fe
 
Code: SS06MO0230H