Johnson, G. L. University of California at Santa Barbara, g_johnso@lifesci.lscf.ucsb.edu
MacIntyre, S. L. UCSB, sally@seaotter.ucsb.edu

 
Physiological Analysis of Photoacclimation in Phytoplankton Utilizing Scales of Mixing Determined from Microstructure Profiles
 
Few physiological studies of phytoplankton have utilized realistic light variations experienced by cells in the water column due to vertical mixing. However, data from microstructure profilers can be used to extrapolate realistic light variations. These profilers measure small-scale conductivity and temperature variations with depth. From these data, the vertical extent of eddies and the time scales of vertical mixing within the water column can be determined. These data can then be used to predict realistic cell trajectories, and, therefore, actual light variations experienced by phytoplankton. Based on these light variations, rates of photoacclimation of two Mono Lake phytoplankton species were determined under both nutrient-rich and nutrient-limited conditions. Cultures of a Diatom (SP) and a Chlorophyte (SP) isolated from Mono Lake, CA were placed in closed containers at various depths for set time periods. Both these parameters were based on realistic mixing values determined from microstructure profiles taken within the lake. The extent of photoacclimation was monitored through changes in xanthophyll cycle pigments and analyzed using HPLC.
 
Day: Tuesday, Feb. 2
Time: 11:30 - 11:45am
Location: Hilton of Santa Fe
 
Code: SS05TU1130H