Kellogg, C. A. University of South Florida, kelloggc@gusun.georgetown.edu
Paul, J. A. University of South Florida, jpaul@seas.marine.usf.edu

 
DEGREE OF UV-DAMAGE AND REPAIR CAPABILITIES ARE RELATED TO G+C CONTENT IN MARINE PHAGES
 
Photoreactivation and excision repair were investigated in two marine Vibrio parahaemolyticus hosts as they catalyzed the reactivation of seven phages. There was no difference in excision repair or background (no repair), but substantial variation was observed in photoreactivation of the viruses. Although sharing a common host, several of the phages had very different survival curves, with survival at the highest energy dose (646 J/m2) ranging from 0.5% to 0.000001%. Photoreactivation was the dominant repair mode in all but one case, although excision alone could increase survival by 1-5 orders of magnitude. Investigation of the G+C content showed the phage with no photoreactivation capacity had a G+C content of 16%. Correlation of the degree of damage to G+C content for six phages yielded a correlation coefficient of 0.955, indicating a strong relationship between the number of thymine dimer targets and a reduced ability to photoreactivate. Evolution of high G+C content may be a strategy for UV protection in phages.
 
Day: Wednesday, Feb. 3
Time: 09:30 - 09:45am
Location: Sweeney Center
 
Code: CS65WE0930S