Pelletier, D. M. Cornell University, dmp25@cornell.edu
Chan, F. M. Cornell University, fc19@cornell.edu
Marino, R. M. Cornell University, rmm3@cornell.edu

 
CONCENTRATING NATURAL ASSEMBLAGES OF PLANKTON CELLS: A TEST OF TANGENTIAL FLOW FILTRATION
 
Many ecological and biogeochemical questions require an ability to concentrate large volumes of live plankton cells. Some previous studies have suggested that Tangential Flow Filtration (TFF) is a useful tool for this, however there are few tests of this approach We tested TFF concentration methods on natural plankton assemblages (<70um) from Narragansett Bay, Rhode Island to ultimately use for stable isotopic analysis. Filtration resulted in poor recovery efficiencies in all cell species counted, particularly in large diatoms (e.g. Ditylum) and chained colonial cells (e.g. Skeletonema). Losses were due to both lysing of cells and adsorption of cells on the filter membrane and was not consistent across species. Because of cell lysing and preferential loss of certain plankton species thus altering the natural assemblage, TFF was not deemed an appropriate method for concentrating plankton cells for isotopic or elemental analysis.
 
Day: Thursday, Feb. 4
Time: Poster
Location: Sweeney Center
 
Code: CS62TH0925S