Shankle, A. M. Scripps Institution of Oceanography, ashankle@ucsd.edu
Franks, P. M. Scripps Institution of Oceanography, pfranks@ucsd.edu
Azam, F. Scripps Institution of Oceanography, fazam@ucsd.edu

 
INTRA-POPULATION GENETIC VARIATION IN THE DINOFLAGELLATES LINGULODINIUM POLYEDRUM AND PROROCENTRUM MICANS EVALUATED USING DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE)
 
Dinoflagellates are important coastal phytoplankton. About 100 species of dinoflagellate can grow to such densities that they form a "red tide." Many of these species are toxic and can cause human illness. However, little is known about the population variability within a species for this taxon. For instance, how many strains are present, and how does the dominance of a strain vary over time and during a bloom? Determination of intra-specific variation is not possible using traditional morphological methods. Studies at this level have been done using physiological variation, enzyme electrophoresis, and, more recently, genetic sequencing. In this study, we used denaturing gradient gel electrophoresis (DGGE) to examine base-pair differences between isolates in an ~700 b.p. sequence of DNA. Individuals were collected, isolated, and cultured from populations of the species Lingulodinium polyedrum and Prorocentrum micans from La Jolla, CA in June and July 1998. The DNA of the isolates was extracted, amplified, and analyzed using DGGE. DGGE results allow us to compare the genetic sequence in the amplified region of the isolates with each other. With these results we determine whether the populations of the dinoflagellates are composed of one or many strains and how the structure changes with time.
 
Day: Thursday, Feb. 4
Time: Poster
Location: Sweeney Center
 
Code: CS62TH0382S