Grembowicz, K. P.. University of Southern Mississippi, firstname.lastname@example.org
Shiller, A. P.. University of Southern Mississippi, email@example.com
VALIDATION OF FLOW FIELD-FLOW FRACTIONATION AS A SUITABLE TOOL FOR CHARACTERIZING FLUVIAL COLLOIDS
Flow field-flow fractionation (FFF) is an elution technique wherein colloidal particles can be separated based on their hydrodynamic diameters. While there have been numerous studies of the application of FFF to the separation of latex beads, proteins, and polymers, only recently has the technique been applied to the characterization of environmental samples. Part of the difficulty in applying flow FFF methods to environmental sample characterization is the dilute nature of samples (relative to the sensitivity of the detectors coupled to the FFF channel) as well as uncertainty as to whether the flow FFF process may itself cause coagulation of colloids. We have been utilizing an on-channel concentration technique that allows us to inject river water samples up to 10 ml in volume on the FFF channel and thereby avoid the problem of dilute samples. Additionally, we have been examining numerous elution conditions (e.g., different carriers and membrane barriers) to investigate potential artifacts when dealing with environmental samples. Discrete fractions from samples separated under differing conditions have been examined by SEM. The results are allowing us to select optimum conditions for flow FFF separation of fluvial colloids and determine the validity of this promising methodology.
Day: Tuesday, Feb. 2
Location: Sweeney Center