Update of “Analysis of ¹⁵N incorporation into D-alanine: A new method for tracing nitrogen uptake by bacteria” (Veuger et al. 2005, Limnol. Oceanogr.: Methods 3: 230-240)

Bart Veuger, Jack J. Middelburg, Henricus T.S. Boschker and Marco Houtekamer
Limnol. Oceanogr. Methods (2007) 5:192-194

After publication of the original manuscript, the presented method has been applied in various ¹⁵N- and ¹³C-labeling studies to trace incorporation of ¹⁵N- and/or ¹³C into bacterial and total microbial biomass in different coastal sediments (Veuger et al. 2006, Veuger 2006). These applications and analysis of the D-Ala content of some algal- and cyanobacterial cultures yielded valuable information regarding the interpretation of ¹⁵N and ¹³C incorporation into D-Ala and other hydrolysable amino acids (HAAs), which is presented in this update.