Nitrogen isotopic response of prokaryotic and eukaryotic phytoplankton to nitrate availability in Sargasso Sea surface waters

Lija A. Treibergs, Sarah E. Fawcett, Michael W. Lomas and Daniel M. Sigman

Limnol. Oceanogr., 59(3), 2014, 972-985 | DOI: 10.4319/lo.2014.59.3.0972

ABSTRACT: Taxon-specific nitrogen (N) isotope data from the summertime Sargasso Sea have previously suggested reliance of prokaryotic phytoplankton on regenerated N but a greater importance of nitrate assimilation for eukaryotic phytoplankton. To investigate this further, particles collected in the summer from ∼ 100 m at the Bermuda Atlantic Time-series Study site were incubated in particle-free, unamended surface seawater (from 4 m and 30 m) with measured ambient nitrate and ammonium concentrations. Preincubation and postincubation particles were sorted using flow cytometry, and the δ15N of separated prokaryotic and eukaryotic phytoplankton was analyzed. In July 2009 and 2010, nitrate was undetectable throughout the euphotic zone (the upper ∼ 100 m). The δ15N of prokaryotic biomass was initially low and remained low upon incubation, with one exception out of six. Preincubation eukaryotic δ15N was higher than or similar to prokaryotic δ15N and decreased or remained low after incubation. These data confirm the expectation that, in nitrate-deplete water, all phytoplankton shift toward a low δ15N characteristic of recycled N assimilation. In June 2010, the euphotic zone nitrate concentration was anomalously high (∼ 1 µmol L−1), and the δ15N of all populations varied as a function of depth, with highest δ15N in the surface where nitrate was lowest. After incubation in surface water, the δ15N of all sorted phytoplankton increased, suggesting nitrate assimilation by all groups, including Prochlorococcus, the nitrate assimilatory capabilities of which have been debated. This study supports the use of the δ15N of sorted phytoplankton as an indicator of the dominant N source fueling their growth.

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