Nitrate uptake in the scleractinian coral Stylophora pistillata

Grover, Renaud, Jean-François Maguer, Denis Allemand, Christine Ferrier-Pagès

Limnol. Oceanogr., 48(6), 2003, 2266-2274 | DOI: 10.4319/lo.2003.48.6.2266

ABSTRACT: We assessed the uptake rates of nitrate by the scleractinian coral Stylophora pistillata by following 15N from seawater into the coral tissue. Two sets of corals were first prepared, with "nitrate-enriched" corals grown in 5 µmol L-1 NO3- and control corals grown in ≤µmol L-1 NO3-. Uptake rates at 0.3 and 3 µmol L-1 [15N]NO3- were then measured. Most of the %15N enrichment occurred in the zooxanthellae fraction. Uptake rates were not significantly different between nitrate-enriched and control corals, suggesting that they were not dependent on a nitrate acclimation. These rates increased with the in situ nitrate concentration and varied from 1.2 ± 0.2 ng h-1 cm-2 N to 6.1 ± 1.1 ng h-1 cm-2 N in the algal fraction at 0.3 and 3 µmol L-1 [15N]NO3-, respectively. In a second experiment, two sets of corals were prepared, with "ammonium-enriched" corals grown in 5 µmol L-1 NH4+ and control corals grown in <1 µmol L-1 NH4+. Uptake rates at 3 µmol L-1 [15N]NO3- were measured. These rates were significantly lower with high NH4+ concentrations in seawater. In the algal fraction, they ranged from 0.1 to 0.6 ng 14 h-1 cm-2 N in NH4+-enriched corals and from 2.2 to 4.5 ng h-1 cm-2 N in control corals. Nitrate can therefore be considered as an important source of nitrogen for corals, at least when ammonium concentrations are low in seawater.

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