CO2 and HCO3- uptake in marine diatoms acclimated to different CO2 concentrations

Burkhardt, Steffen, Gabi Amoroso, Ulf Riebesell, Dieter Sültemeyer

Limnol. Oceanogr., 46(6), 2001, 1378-1391 | DOI: 10.4319/lo.2001.46.6.1378

ABSTRACT: Rates of cellular uptake of CO2 and HCO3- during steady-state photosynthesis were measured in the marine diatoms Thalassiosira weissflogii and Phaeodactylum tricornutum, acclimated to CO2 partial pressures of 36, 180, 360, and 1,800 ppmv. In addition, in vivo activity of extracellular (eCA) and intracellular (iCA) carbonic anhydrase was determined in relation to CO2 availability. Both species responded to diminishing CO2 supply with an increase in eCA and iCA activity. In P. tricornutum, eCA activity was close to the detection limit at higher CO2 concentrations. Simultaneous uptake of CO2 and HCO3- was observed in both diatoms. At air-equilibrated CO2 levels (360 ppmv), T. weissflogii took up CO2 and HCO3- at approximately the same rate, whereas CO2 uptake exceeded HCO3- uptake by a factor of two in P. tricornutum. In both diatoms, CO2 :HCO3- uptake ratios progressively decreased with decreasing CO2 concentration, whereas substrate affinities of CO2 and HCO3- uptake increased. Half-saturation concentrations were always <=5 mM CO2 for CO2 uptake and <700 mM HCO3- for HCO3- uptake. Our results indicate the presence of highly efficient uptake systems for CO2 and HCO3- in both diatoms at concentrations typically encountered in ocean surface waters and the ability to adjust uptake rates to a wide range of inorganic carbon supply.

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