Nanoscale patchiness of bacteria in lake water studied with the spatial information preservation method
Limnol. Oceanogr., 43(2), 1998, 307-314 | DOI: 10.4319/lo.1998.43.2.0307
Abstract: Direct evidence that free-living bacteria can form small patches in the water column has been unavailable, despite long-standing hypotheses, because traditional sampling methods destroy the aggregations. The recently developed spatial information preservation (SIP) method sufficiently preserves the two-dimensional, relative spatial distribution of aquatic microorganisms to resolve patches on the scale of tens to hundreds of micrometers (nanoscale patchiness). We collected samples from lake water using the SIP method. Nanoscale patches were not found in several unmanipulated lake samples. However, we were able to stimulate patch formation by filtering out organisms larger than