| SS4.02 Ecology and Physiology of Marine Organisms: Insights from Genes, Genomes, and Proteomes |
| Chistoserdov, A, Y, University of Louisiana at Lafayette, Lafayette, USA, ayc6160@louisiana.edu |
| Madrid, V, M, Stony Brook University, Stony Brook, USA, vmadrida@ic.sunysb.edu |
| Aller, J, Y, Stony Brook University, Stony Brook, USA, jyaller@notes.cc.sunysb.edu |
| Aller, R, C, Stony Brook University, Stony Brook, USA, raller@notes.cc.sunysb.edu |
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| PHYLOGENETIC DIVERSITY OF RIBULOSE-1,5-BIPHOSPHATE CARBOXYLASE/OXYGENASE GENES OF MICROBIAL COMMUNITIES FROM MOBILE DELTAIC SEDIMENTS |
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| Autotrophic CO2 fixation via the Calvin cycle was studied by amplification and cataloging of the genes for ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO, forms I and II) for 10-20 and >50cm depth intervals from tropical suboxic, deltaic muds along French Guiana and in the Gulf of Papua. Here, we report the results of comparative sequence analysis of RubisCOs form I and II genes from closely and distantly related autotrophic organisms. Sequence analysis showed that retrieved sequences have the characteristic RuBisCO amino acid motif that exists in other RuBisCOs. The phylogenetic affiliation of the most common cbbL and cbbM sequences were closely related to forms I and II of cultured and uncultured chemoautolithotrophic alpha-, beta- and gamma proteobacteria. RuBisCO genes closely related to Thiobacillus spp. appear to be dominant in this environment. The presence of RuBisCO genes in microbial communities from suboxic sediments indicates a net production of CO2 and the probable bacterial participation in the sequestration of CO2. |
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