Aquatic Sciences Meeting, Albuquerque 2001

PC18 Biogeochemical Cycles

Date: Thursday, February 15, 2001

Location: Southwest Hall

Svensson, E, , University of Connecticut, Groton, CT, USA, elisabeth.svensson@uconn.edu

Skoog, A, , University of Connecticut, Groton, CT, USA, annelie.skoog@uconn.edu

SEPARATION AND DETECTION OF LOW MOLECULAR WEIGHT ORGANIC ACIDS IN SEAWATER USING HPLC WITH PDA/UV AND FLUORESCENCE DETECTION.

A semi-automated method for separation and detection of low molecular weight (LMW) organic acids at seawater concentrations is presented. Derivatization of the organic acids with a fluorescent label makes possible the use of fluorescence detection, thereby lowering the detection limit to the nanomolar range. Liquid chromatography and a tandem unit consisting of a photodiode array-detector and a fluorescent detector are used for quantification. Blank and recovery data will also be presented.

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Aquatic Sciences Meeting, Albuquerque 2001

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