Aquatic Sciences Meeting, Albuquerque 2001
| PC08 New Techniques and Technologies from Single Cells to the Global Ocean |
| Date: Wednesday, February 14, 2001 |
| Location: Southwest Hall |
| |
| Twining, B, S, Marine Sciences Research Center, SUNY, Stony Brook, USA, btwining@ic.sunysb.edu |
| Baines, S, B, Marine Sciences Research Center, SUNY, Stony Brook, USA, sbaines@ms.cc.sunysb.edu |
| Fisher, N, S, Marine Sciences Research Center, SUNY, Stony Brook, USA, nfisher@notes.cc.sunysb.edu |
| Jacobsen, C, , Department of Physics and Astronomy, SUNY, Stony Brook, USA, jacobsen@xray1.physics.sunysb.edu |
| Maser, J, , Advanced Photon Source, Argonne National Laboratory, Argonne, USA, maser@aps.anl.gov |
| |
| A NOVEL TECHNIQUE FOR THE QUANTIFICATION AND LOCALIZATION OF TRACE ELEMENTS IN PLANKTON: X-RAY FLUORESCENCE MICROPROBE ANALYSIS |
 |
| Efforts to measure trace elements in plankton generally rely on analyses of size-fractionated bulk particles, but this method cannot distinguish between different types of similarly sized particles or measure metal concentrations in individual cells. We describe a new approach to the quantification of trace elements in individual plankton cells using an x-ray fluorescence microprobe at a high-energy synchrotron source. Diverse nanoplankton cells collected off Southampton, NY were mounted on EM grids, the distributions of elements in specific cells were mapped, and metal concentrations were normalized to dry wt. Results indicate that ciliate and diatom samples have similar Fe and Cu concentrations, but diatoms have higher Si, Mn, and Zn levels. Nanoflagellates have lower concentrations of these 5 elements than larger ciliates and diatoms, with levels within the range of published values. Element concentrations were similar between replicate ciliates, diatoms, and flagellates. This approach is capable of measuring Mn, Fe, Zn, Cu, Cr, Ni, Se, and other metals in individual cells at ppb levels and generally shows promise for quantifying trace elements in natural plankton assemblages. |
| |
| This Session Listing
|
Home | Information | Employment | Education | Meetings | Policy | Publications | Students | Forms | Search
Copyright © 2002 American Society of Limnology and Oceanography. All Rights Reserved
