Aquatic Sciences Meeting, Albuquerque 2001

CS20 Microbial Dynamics

Date: Friday, February 16, 2001, Time: 11:30:00 AM

Location: Ruidoso/Pecos

Holcombe, B, L, University of Washington, Seattle, USA, holcombe@ocean.washington.edu

Keil, R, G, University of Washington, Seattle, USA, rickkeil@u.washington.edu

BACTERIAL DEGRADATION OF PROTIEN UNDER DIFFERENT REDOX CONDITIONS

Protein is the most biologically active reservoir of carbon found in the ocean, however little is known about its reactivity under different redox conditions nor its reactivity relative to other, similar sized, substrates. To examine these questions oxic, sub-oxic, and anoxic water column samples were incubated under their respective conditions. Replicate incubations from each condition were fed different ratios of protein to carbohydrate (100:0, 50:50, 25:75, 0:0) to examine how substrate availability affects degradation kinetics. Simultaneous measurements of DOC, DON, and total protein were made to monitor the progress of protein and total DOM degradation. The reactivity of bulk DOC differs from the protein reactivity under all three environmental conditions. It was also observed that the type of substrate available dictates the relative reactivity rate of protein. Understanding more about the reactivity of protein under various conditions can help decipher the ultimate fate of DOM.

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Aquatic Sciences Meeting, Albuquerque 2001

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