Aquatic Sciences Meeting, Albuquerque 2001

PC03 Undergraduate Education

Date: Tuesday, February 13, 2001

Location: Southwest Hall

Knight, R, F, Old Dominion University, Norfolk, USA, rknig001@odu.edu

Gordon, A, S, Old Dominion Univ., Norfolk, USA, Agordon@odu.edu

Consolvo III, J, A, Old Dominion Univ., Norfolk, USA, jconsolv@odu.edu

Dryden, C, L, Old Dominion Univ., Norfolk, USA, cdryden@odu.edu

ISOLATION AND CHARACTERIZATION OF COPPER COMPLEXING LIGANDS FROM ESTUARINE WATERS

Copper-complexing ligands are important determinants of copper bioavailability in natural waters. In this study we used chromatographic techniques to isolate copper-complexing ligands from estuarine waters. Although L1-class ligands were not isolated in this study, significant progress was made. The samples that were studied were obtained using an IMAC column to "trap" the copper-binding material in the estuarine water. The resulting material was then assayed for protein and polysaccharide concentration. The protein assays showed that there was a measurable amount of protein in the samples analyzed. The polysaccharide assays showed a very small and almost insignificant amount of polysaccharide in the samples. IMAC fractions were further separated by reverse-phase HPLC and selected fractions were assayed for copper complexing activity.

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Aquatic Sciences Meeting, Albuquerque 2001

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