Aquatic Sciences Meeting, Albuquerque 2001
| PC17 Phytoplankton Physiology and Ecology |
| Date: Thursday, February 15, 2001 |
| Location: Southwest Hall |
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| Heil, C, A, University of South Florida, College of Marine Science, St. Petersburg, FL, USA, cheil@seas.marine.usf.edu |
| Vargo, G, A, University of South Florida, College of Marine Science, St. Petersburg, FL, USA, vargo@seas.marine.usf.edu |
| Lester, K, , University of South Florida, College of Marine Science, St. Petersburg, FL, USA, klester@seas.marine.usf.edu |
| Merkt, R, , University of South Florida, College of Marine Science, St. Petersburg, FL, USA, rmerkt@seas.marine.usf.edu |
| Neely, M, B, University of South Florida, College of Marine Science, St. Petersburg, FL, USA, mneely@seas.marine.usf.edu |
| Spence, D, , University of South Florida, College of Marine Science, St. Petersburg, Fl, USA, dspence@seas.marine.usf.edu |
| Walsh, J, J, University of South Florida, College of Marine Science, St. Petersburg, FL, USA, jwalsh@seas.marine.usf.edu |
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| A comparison of two fluorometeric methods for determining chlorophyll a on the West Florida Shelf. |
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| Two methods for measuring chlorophyll a (Chl a), with associated phaeopigments (Holm-Hansen & Riemann 1978) and in the presence of chlorophyll b (Chl b) (Welschmeyer 1994), were compared in 2124 samples from West Florida shelf waters. Results of the two methods were significantly correlated (r2=0.989), with the Welschmeyer method consistently producing an average of 17.2 (+/-0.3)% higher Chl a in 97.3% of all samples over a range from 0.01 to 29.2 ug Chl a/l. This trend encompassed different phytoplankton populations (e.g. Gymnodinium breve and diatom blooms, the deep chlorophyll a maximum (DCM) and oligotrophic (Chl a< 0.1 ug/l) waters) and did not vary with season. In nearshore diatom and dinoflagellate dominated populations, the difference between methods was related to phaeopigment interference, while differences with DCM and oligotrophic water samples were related to a combination of interference from both phaeopigments and Chl b. Our results suggest that measurements of Chl a made with the Holm-Hansen and Riemann method underestimate true Chl a concentrations in both coastal and offshore waters. |
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