Aquatic Sciences Meeting, Albuquerque 2001
| SS04 Environmental Microbial Genomics (Environmental and Disciplinary Connections) |
| Date: Wednesday, February 14, 2001, Time: 3:30:00 PM |
| Location: Brazos |
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| Murray, A, E, Center for Microbial Ecology, Michigan State University, East Lansing, USA, murraya@msu.edu |
| Lies, D, , California Institute of Technology, Pasadena, USA, docdpl@yahoo.com |
| Li, G, , Oak Ridge National Laboratory, Oak Ridge, USA, |
| Nealson, K, , Jet Propulsion Laboratory, Pasadena, USA, Kenneth.H.Nealson@jpl.nasa.gov |
| Zhou, J, , Oak Ridge National Laboratory, Oak Ridge, USA, ytz@ornl.gov |
| Tiedje, J, M, Center for Microbial Ecology, Michigan State University, East Lansing, USA, tiedjej@msu.edu |
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| ADVANCES IN MICROARRAY TECHNOLOGY: ESTABLISHING CONNECTIONS IN MICROBIAL ECOLOGY AND EVOLUTION. |
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| DNA microarrays offer a platform in which nucleic acids are deposited in a highly ordered and parallel format. There are numerous applications of this technology to the study of microorganisms extending from understanding differential gene expression under varying environmental conditions to studying genomic homology between closely related organisms at a high level of resolution. Microarrays constructed with 1000 Shewanella oneidensis MR1 genes have been used in experiments aimed at detecting gene expression for Shewanella oneidensis, MR-1 under different electron accepting conditions. Attempts to quantify differential gene expression will be described, and resulting biogeochemical and environmental implications explored. In addition to studying gene expression, DNA microarrays have been used to determine the level of genomic relatedness between members of the Shewanella genus. DNA microarray hybridizations afford the ability to understand relatedness on a gene by gene level, so that detailed patterns of relatedness can be determined for organisms that are closely related (>96% rRNA sequence homology). The future of microbial biology, ecology, taxonomy, physiology and evolution promise to be significantly impacted by the development and applications of microarray technology. |
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